A1: What is the difference between a UV-Visible spectrophotometer and a visible spectrophotometer?
Q1: The difference between UV-Visible spectrophotometer and visible spectrophotometer is that the measurement wavelength range is different, UV generally uses a hydrogen lamp, the measurement wavelength range is of 180 ~ 350NM, visible generally uses a tungsten lamp, and the measurement wavelength range of 320 ~ 1000NM. The so-called UV-Visible spectrophotometer also means that this instrument can change the light source, can determine the absorption peak in the UV and visible part of the compound.
A2: What is the wavelength range of the UV-Visible spectrophotometer?
Q2: Ultraviolet-visible (UV-Vis) spectrophotometers use a light source that illuminates the sample with light in the UV to visible wavelength range (typically 190 to 900 nm). UV-Vis-NIR spectrophotometers extend the wavelength range to the near-infrared (NIR) (800 to 3200 nm).
A3: What is the principle of operation of a spectrophotometer?
Q3: The basic principle of the spectrophotometer is that the substances in solution are excited by light irradiation, resulting in the effect of light absorption, the absorption of light by substances is selective, a variety of different substances have their own absorption spectrum, so when a monochromatic light through the solution, its energy will be absorbed and diminished, the degree of light energy diminution and the concentration of substances have a certain proportional relationship, that is, in line with the colorimetric principle - -Biel's law.
Tips: How does a single-beam spectrophotometer work?
A single-beam spectrophotometer determines color by measuring the intensity of the light source before and after it is inserted into the test sample. The light source is modulated (turned on and off) to distinguish between light from the light source and light from the flame.